THO复合体亚基1 (核基质蛋白p84) (p84N5) (hTREX84)
THO复合体亚基1 (核基质蛋白p84) (p84N5) (hTREX84)
Q96FV9功能描述
TREX复合物中THO亚复合物的组分，该复合物被认为偶联mRNA转录、加工和核输出，且特异性结合剪接后的mRNA而非未剪接的前体mRNA (PubMed:15833825, PubMed:15998806, PubMed:17190602)。多聚腺苷酸化RNA的有效输出所必需 (PubMed:23222130)。仅THOC1-THOC2-THOC3核心复合物即足以结合输出因子NXF1-NXT1并促进DDX39B/UAP56的ATP酶活性 (PubMed:33191911)。TREX通过不依赖转录的机制被募集到剪接后的mRNA上，结合外显子连接复合物(EJC)上游的mRNA，并以依赖剪接和帽结构的方式被募集到mRNA 5'端附近的区域，在那里通过TAP/NXF1途径发挥mRNA输出至细胞质的功能 (PubMed:15833825, PubMed:15998806, PubMed:17190602)。调节一部分基因的转录延伸 (PubMed:22144908)。通过防止共转录R-loop形成参与维持基因组稳定性 (By similarity)。可能在毛细胞形成中发挥作用，因此可能参与听觉 (By similarity)。 {ECO:0000250|UniProtKB:Q7SYB2, ECO:0000269|PubMed:15833825, ECO:0000269|PubMed:15998806, ECO:0000269|PubMed:17190602, ECO:0000269|PubMed:22144908, ECO:0000269|PubMed:23222130, ECO:0000269|PubMed:33191911}.; FUNCTION: 参与以激活caspase-6、增加BAK1和BCL2L1表达以及激活NF-kappa-B为特征的细胞凋亡途径。该途径不需要p53/TP53，p53/TP53的存在也不影响细胞杀伤效率。在凋亡开始前激活G2/M细胞周期检查点。与RB1结合可抑制细胞凋亡。; FUNCTION: (微生物感染) TREX复合物对于卡波西肉瘤相关疱疹病毒(KSHV)无内含子mRNA的输出和传染性病毒的产生至关重要。 {ECO:0000269|PubMed:18974867}.
组织特异性
Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression.
亚细胞定位
[Isoform 1]: Nucleus speckle. Nucleus, nucleoplasm. Nucleus matrix. Cytoplasm. Note=Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm.
关键词