丝氨酸/苏氨酸蛋白激酶PLK3 (EC 2.7.11.21) (细胞因子诱导的丝氨酸/苏氨酸蛋白激酶) (FGF诱导激酶) (Polo样激酶3) (PLK-3) (增殖相关激酶)
丝氨酸/苏氨酸蛋白激酶PLK3 (EC 2.7.11.21) (细胞因子诱导的丝氨酸/苏氨酸蛋白激酶) (FGF诱导激酶) (Polo样激酶3) (PLK-3) (增殖相关激酶)
Q9H4B4功能描述
丝氨酸/苏氨酸蛋白激酶，参与细胞周期调控、应激反应和高尔基体解体。Polo样激酶通过结合并磷酸化已被磷酸化的蛋白质起作用，这些蛋白质具有被POLO box结构域识别的特定基序。磷酸化ATF2、BCL2L1、CDC25A、CDC25C、CHEK2、HIF1A、JUN、p53/TP53、p73/TP73、PTEN、TOP2A和VRK1。参与细胞周期调控：进入S期和胞质分裂所必需。磷酸化BCL2L1，从而调节有丝分裂期间的G2检查点和胞质分裂进程。在应激反应中起关键作用：在应激刺激下迅速被激活，如电离辐射、活性氧（ROS）、高渗应激、UV照射和缺氧。通过磷酸化CDC25A、p53/TP53和p73/TP73参与DNA损伤反应和G1/S期转换检查点。响应活性氧（ROS）磷酸化p53/TP53，从而促进p53/TP53介导的细胞凋亡。响应DNA损伤磷酸化CHEK2，促进G2/M期转换检查点。响应DNA损伤磷酸化转录因子p73/TP73，从而抑制p73/TP73介导的转录激活和促凋亡功能。响应缺氧磷酸化HIF1A和JUN。角膜上皮细胞在高渗应激后磷酸化ATF2。也参与细胞周期中的高尔基体解体：作为MEK1/MAP2K1依赖途径的一部分，通过介导VRK1的磷酸化诱导有丝分裂期间的高尔基体断裂。可能通过其与CIB1的相互作用参与核内有丝分裂细胞周期，这是一种核分裂和胞质分裂均被中断的有丝分裂形式，是巨核细胞分化的标志。 {ECO:0000269|PubMed:10557092, ECO:0000269|PubMed:11156373, ECO:0000269|PubMed:11447225, ECO:0000269|PubMed:11551930, ECO:0000269|PubMed:11971976, ECO:0000269|PubMed:12242661, ECO:0000269|PubMed:14968113, ECO:0000269|PubMed:14980500, ECO:0000269|PubMed:15021912, ECO:0000269|PubMed:16478733, ECO:0000269|PubMed:16481012, ECO:0000269|PubMed:17264206, ECO:0000269|PubMed:17804415, ECO:0000269|PubMed:18062778, ECO:0000269|PubMed:18650425, ECO:0000269|PubMed:19103756, ECO:0000269|PubMed:19490146, ECO:0000269|PubMed:20889502, ECO:0000269|PubMed:20940307, ECO:0000269|PubMed:20951827, ECO:0000269|PubMed:21098032, ECO:0000269|PubMed:21264284, ECO:0000269|PubMed:21376736, ECO:0000269|PubMed:21840391, ECO:0000269|PubMed:9353331}.
组织特异性
Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor.
亚细胞定位
Cytoplasm. Nucleus. Nucleus, nucleolus. Golgi apparatus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Translocates to the nucleus upon cisplatin treatment. Localizes to the Golgi apparatus during interphase. According to a report, PLK3 localizes only in the nucleolus and not in the centrosome, or in any other location in the cytoplasm (PubMed:17264206). The discrepancies in results may be explained by the PLK3 antibody specificity, by cell line-specific expression or post-translational modifications.
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