类核酸内切酶8 3 (EC 3.2.2.-) (EC 4.2.99.18) (DNA糖基化酶FPG2) (DNA糖基化酶/AP裂解酶Neil3) (类核酸内切酶VIII 3) (Nei样蛋白3)
类核酸内切酶8 3 (EC 3.2.2.-) (EC 4.2.99.18) (DNA糖基化酶FPG2) (DNA糖基化酶/AP裂解酶Neil3) (类核酸内切酶VIII 3) (Nei样蛋白3)
Q8TAT5功能描述
DNA糖基化酶，优先作用于单链DNA (ssDNA)，或部分ssDNA结构（如气泡和叉结构），而非双链DNA (dsDNA) (PubMed:12433996, PubMed:19170771, PubMed:22569481, PubMed:23755964)。介导响应复制压力的链间交联修复：通过介导DNA糖基化酶活性发挥作用，切割构成链间交联的两个N-糖苷键之一，从而避免双链断裂的形成，但产生了一个可被跨损伤合成聚合酶绕过的无碱基位点 (By similarity)。在体外，对单链DNA和双链DNA中的乙内酰脲损伤螺咪唑并乙内酰脲 (Sp) 和鸟嘌呤乙内酰脲 (Gh) 显示出强糖基化酶活性；同时也识别单链DNA中的FapyA、FapyG、5-OHU、5-OHC、5-OHMH、Tg和8-oxoA损伤 (PubMed:12433996, PubMed:19170771, PubMed:22569481, PubMed:23755964)。未检测到对8-oxoG的活性 (PubMed:12433996, PubMed:19170771, PubMed:22569481, PubMed:23755964)。亦显示较弱的DNA-(无嘌呤或无嘧啶位点)裂解酶活性 (PubMed:12433996, PubMed:19170771, PubMed:22569481, PubMed:23755964)。在体内，似乎是新生儿组织中从ssDNA上去除Sp和Gh的主要酶 (PubMed:12433996, PubMed:19170771, PubMed:22569481, PubMed:23755964)。 {ECO:0000250|UniProtKB:A0A1L8HU22, ECO:0000269|PubMed:12433996, ECO:0000269|PubMed:19170771, ECO:0000269|PubMed:22569481, ECO:0000269|PubMed:23755964}。
组织特异性
Expressed in keratinocytes and embryonic fibroblasts (at protein level). Also detected in thymus, testis and fetal lung primary fibroblasts.
亚细胞定位
Nucleus
关键词