DDB2 Q92466

DNA损伤结合蛋白2 (DDB p48亚基) (DDBb) (损伤特异性DNA结合蛋白2) (UV损伤DNA结合蛋白2) (UV-DDB 2)

功能描述

该Protein分别作为UV-DDB复合物和DCX (DDB1-CUL4-X-box) 复合物的一部分，参与DNA修复和protein泛素化 (PubMed:10882109, PubMed:11278856, PubMed:11705987, PubMed:12732143, PubMed:15882621, PubMed:16473935, PubMed:18593899, PubMed:32789493, PubMed:9892649)。UV-DDB复合物 (UV-damaged DNA-binding protein complex) 的核心成分，该复合物识别UV诱导的DNA损伤，并招募核苷酸切除修复通路 (the NER pathway) 的protein以启动DNA修复 (PubMed:10882109, PubMed:11278856, PubMed:11705987, PubMed:12944386, PubMed:14751237, PubMed:16260596, PubMed:32789493)。UV-DDB复合物优先结合环丁烷嘧啶二聚体 (CPD)、6-4光产物 (6-4 PP)、脱嘌呤位点和短错配 (PubMed:10882109, PubMed:11278856, PubMed:11705987, PubMed:12944386, PubMed:16260596)。还作为DCX (DDB2-CUL4-X-box) E3 ubiquitin-protein ligase复合物DDB2-CUL4-ROC1 (也称为CUL4-DDB-ROC1和CUL4-DDB-RBX1) 的底物识别模块发挥作用 (PubMed:12732143, PubMed:15882621, PubMed:16473935, PubMed:18593899, PubMed:26572825)。DDB2-CUL4-ROC1复合物可能在UV诱导的DNA损伤位点泛素化组蛋白H2A、组蛋白H3和组蛋白H4 (PubMed:16473935, PubMed:16678110)。组蛋白的泛素化可能促进它们从核小体中移除，进而促进随后的DNA修复 (PubMed:16473935, PubMed:16678110)。DDB2-CUL4-ROC1复合物也泛素化XPC，这可能增强XPC的DNA结合能力并促进NER (PubMed:15882621)。DDB2-CUL4-ROC1复合物还响应DNA损伤泛素化KAT7/HBO1，导致其降解：识别被ATR磷酸化后的KAT7/HBO1 (PubMed:26572825)。 {ECO:0000269|PubMed:10882109, ECO:0000269|PubMed:11278856, ECO:0000269|PubMed:11705987, ECO:0000269|PubMed:12732143, ECO:0000269|PubMed:12944386, ECO:0000269|PubMed:14751237, ECO:0000269|PubMed:15882621, ECO:0000269|PubMed:16260596, ECO:0000269|PubMed:16473935, ECO:0000269|PubMed:16678110, ECO:0000269|PubMed:18593899, ECO:0000269|PubMed:26572825, ECO:0000269|PubMed:32789493, ECO:0000269|PubMed:9892649}.; FUNCTION: [Isoform D1]: 抑制UV损伤DNA修复。 {ECO:0000269|PubMed:14751237}.; FUNCTION: [Isoform D2]: 抑制UV损伤DNA修复。 {ECO:0000269|PubMed:14751237}.

组织特异性

Ubiquitously expressed; with highest levels in corneal endothelium and lowest levels in brain. Isoform D1 is highly expressed in brain and heart. Isoform D2, isoform D3 and isoform D4 are weakly expressed.

亚细胞定位

Nucleus