LARS1 Q9P2J5

亮氨酸--tRNA连接酶，细胞质 (EC 6.1.1.4) (亮氨酰-tRNA合成酶) (LeuRS) (cLRS)

功能描述

氨酰-tRNA合成酶，催化亮氨酸特异性连接到其同源tRNA (tRNA(Leu))上 (PubMed:25051973, PubMed:32232361)。它通过两步反应进行tRNA氨酰化：Leu首先被ATP激活形成亮氨酰-腺苷酸(Leu-AMP)中间体；随后亮氨酰基团被转移到tRNA受体3'端生成亮氨酰-tRNA (PubMed:25051973)。为了提高催化反应的保真度，它还能够水解错误激活的氨酰-腺苷酸中间体（转移前编辑）和错误 charged 的氨酰-tRNA（转移后编辑）(PubMed:25051973)。 {ECO:0000269|PubMed:19426743, ECO:0000269|PubMed:25051973, ECO:0000269|PubMed:32232361}。 (Self-correction on "mischarged"): "错误 charged" is unprofessional. "错误氨酰化" or "错误装载" is better. "Mischarged aminoacyl-tRNAs" -> "错误氨酰化的氨酰-tRNA" or simply "错误装载的氨酰-tRNA". Let's use "错误氨酰化的氨酰-tRNA" for precision. Re-evaluating "hydrolyze misactivated... and mischarged...": "能够水解错误激活的氨酰-腺苷酸中间体（转移前编辑）和错误氨酰化的氨酰-tRNA（转移后编辑）" Final Polish: FUNCTION: 氨酰-tRNA合成酶，催化亮氨酸特异性连接到其同源tRNA (tRNA(Leu))上 (PubMed:25051973, PubMed:32232361)。它通过两步反应进行tRNA氨酰化：Leu首先被ATP激活形成亮氨酰-腺苷酸(Leu-AMP)中间体；随后亮氨酰基团被转移到tRNA受体3'端生成亮氨酰-tRNA (PubMed:25051973)。为了提高催化反应的保真度，它还能够水解错误激活的氨酰-腺苷酸中间体（转移前编辑）和错误氨酰化的氨酰-tRNA（转移后编辑）(PubMed:25051973)。 {ECO:0000269|PubMed:19426743, ECO:0000269|PubMed:25051973, ECO:0000269|PubMed:32232361}。 One detail: "Leu is initially activated..." -> "Leu" in the source is the abbreviation. I can keep "Leu" or use "亮氨酸". Since I kept "tRNA", keeping "Leu" is acceptable, but translating it to "亮氨酸" flows better in Chinese text. However, the source text specifically uses "Leu" in the second sentence while "leucine" in the first. I will translate "Leu" to "亮氨酸" for clarity but keep "Leu-AMP" as the technical term. Revised sentence 2: 亮氨酸首先被ATP激活形成亮氨酰-腺苷酸(Leu-AMP)中间体；随后亮氨酰基团被转移到tRNA受体3'端生成亮氨酰-tRNA (PubMed:25051973)。 This looks solid. Final check of requirements: 1. Terms: 氨酰-tRNA合成酶, 亮氨酸, 同源tRNA, 氨酰化, 腺苷酸, 中间体, 保真度, 水解, 转移前编辑, 转移后编辑. (All professional). 2. {ECO...} preserved. 3. (PubMed...) preserved. 4. Format preserved. 5. No explanations. Result: FUNCTION: 氨酰-tRNA合成酶，催化亮氨酸特异性连接到其同源tRNA (tRNA(Leu))上 (PubMed:25051973, PubMed:32232361)。它通过两步反应进行tRNA氨酰化：亮氨酸首先被ATP激活形成亮氨酰-腺苷酸(Leu-AMP)中间体；随后亮氨酰基团被转移到tRNA受体3'端生成亮氨酰-tRNA (PubMed:25051973)。为了提高催化反应的保真度，它还能够水解错误激活的氨酰-腺苷酸中间体（转移前编辑）和错误氨酰化的氨酰-tRNA（转移后编辑）(PubMed:25051973)。 {ECO:0000269|PubMed:19426743, ECO:0000269|PubMed:25051973, ECO:0000269|PubMed:32232361}。

亚细胞定位

Cytoplasm